来自可再生油的塑料单体的生物合成
Wenhua Lu1, Jon E Ness, Wenchun Xie
1Polytechnic Institute of New York University, 6 Metrotech Center, Brooklyn, New York 11201, United States.
Journal of the American Chemical Society
|October 13, 2010
概括
工程酵母产生欧米茄-基脂肪酸,这是生物基塑料的关键单体. 这一突破提供了一条具有成本效益的途径,以从可再生资源获得可持续的聚乙烯类材料.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 聚合物科学 聚合物科学
背景情况:
- 欧米茄-基脂肪酸是创建生物基聚乙烯类塑料的理想单体.
- 目前这些酸的有机合成方法昂贵且低效,限制了它们在大规模应用中的使用.
研究的目的:
- 为了设计一种酵母菌株,Candida tropicalis,用于商业上可行的omega-hydroxyfatty acid的生产.
- 建立一个可持续的生物催化平台,从可再生原料中生产有价值的化学品.
主要方法:
- 通过识别和删除16个基因,对Candida tropicalis进行基因工程.
- 向的基因删除包括编码P450细胞染色体,脂肪酒精氧化酶和酒精脱酶的基因删除.
- 通过表达特定的P450酶,证明了各种脂肪酸的高效氧化.
主要成果:
- 通过使用工程化C. tropicalis.实现了商业上可行的欧米茄-基脂肪酸产量.
- 成功修改了酵母菌株以提高脂肪酸氧化效率.
- 展示了该菌株在氧化不同链长度和不和度的脂肪酸方面的多功能性.
结论:
- 工程化C. tropicalis提供了一个有前途的生物催化平台,用于生产欧米茄-基脂肪酸.
- 这种方法为生物基塑料的传统化学合成提供了一种可持续且具有成本效益的替代方案.
- 开发的酵母菌株有助于从可再生资源生产商品化学品.
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