非肌肉肌肉蛋白IIA是简单疹病毒-1的功能性入口受体
Jun Arii1, Hideo Goto, Tadahiro Suenaga
1Division of Viral Infection, Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
Nature
|October 15, 2010
概括
非肌肉髓重链IIA (NMHC-IIA) 作为简单疹病毒-1 (HSV-1) 进入受体,与糖蛋白B (gB) 相互作用. 这一发现揭示了针对HSV-1感染的抗病毒药物开发的新目标.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 简单疹病毒-1 (HSV-1) 导致终身的人类感染,从粘膜皮肤疾病到致命脑炎.
- HSV-1 进入宿主细胞取决于包糖蛋白B (gB) 和D (gD) 的细胞受体.
- 介导HSV-1广泛宿主范围和体内感染的特定gB受体仍然未确定.
研究的目的:
- 为了识别HSV-1糖蛋白B (gB) 的细胞受体.
- 研究非肌肉肌肉蛋白重链IIA (NMHC-IIA) 在HSV-1输入中的作用.
- 探索NMHC-IIA作为抗病毒疗法的潜在目标.
主要方法:
- 在HSV-1耐药细胞中过度表达NMHC-IIA.
- 使用对NMHC-IIA抗体阻止HSV-1感染.
- 在允许细胞中对NMHC-IIA的抑制.
- 评估由HSV-1糖蛋白介导的细胞融合.
- 研究NMHC-IIA细胞表面表达的动态.
- 在细胞培养和小鼠模型中使用肌轻链激酶抑制剂.
主要成果:
- 非肌肉肌肉胺重链IIA (NMHC-IIA) 通过与gB.交互作为HSV-1入口受体.
- 过度表达NMHC-IIA显著增加了耐药细胞中的HSV-1敏感性.
- 对NMHC-IIA和NMHC-IIA抗体的抗体抑制了HSV-1感染.
- 在HSV-1进入初期,NMHC-IIA细胞表面表达迅速被诱导.
- 抑制NM-IIA调节可以在体外和体内减少HSV-1感染.
结论:
- NMHC-IIA被确定为一个功能HSV-1入口受体,与gB.交互.
- 在体外和体内,NMHC-IIA调解了广泛的HSV-1感染性.
- 调节NM-IIA对HSV-1感染至关重要,因为它具有新的抗病毒药物标.
相关概念视频
Herpes
Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
Overview of Myosin Structure and Function
Myosins are a family of molecular motor proteins, first identified in the skeletal muscles, where they are responsible for muscle contraction. Along with their role in muscle contraction, these proteins also play a role in the intracellular transport of molecules and vesicles. There are twenty-four classes of myosins based on their domain sequence and organization. Of the twenty-four, six classes (Myosin I, Myosin II, Myosin V, Myosin VI, Myosin VII, and Myosin X) have been well characterized.
Intracellular Movement of Viruses and Bacteria
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a virus that...
Role of Myosin in Cell Migration
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Inhibitors Of Virion Release
Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Rabies
Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...


