子结合复杂子单元是需要拼接Drosophila MAP激酶,一个大的异色染色基因基因
Jean-Yves Roignant1, Jessica E Treisman
1Kimmel Center for Biology and Medicine of the Skirball Institute, NYU School of Medicine, Department of Cell Biology, 540 First Avenue, New York, NY 10016, USA.
Cell
|October 16, 2010
概括
异构体结合复合体 (EJC) 子单元Mago nashi (mago) 对于MAPK基因拼接至关重要. 失去mago通过损害表皮生长因子受体信号传递来破坏光受体发育.
科学领域:
- 分子生物学分子生物学
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 子结合复合体 (EJC) 是一个组装在拼接mRNA上的蛋白质复合体.
- EJC在mRNA翻译,局部化和降解中发挥作用.
- 在EJC组件中的突变可能导致发育缺陷.
研究的目的:
- 调查Drosophila mago nashi (mago) 的功能,这是一个核心的EJC子单元.
- 了解mago在光受体分化和基因表达中的作用.
主要方法:
- 在Drosophila中隔离了mago突变.
- 对MAPK mRNA水平和拼接模式的分析.
- 对表皮生长因子受体信号的评估.
- 研究mago对培养细胞中异色基因表达的影响.
主要成果:
- 马戈的损失特别影响光受体的分化.
- 巨枯竭会降低MAPK mRNA水平,并损害表皮生长因子受体信号传递.
- 为了正确的MAPK拼接,需要Mago,而不是转录或稳定性.
- 马戈敲击不成比例地影响了异性染色体中的大型基因.
结论:
- 马戈在特定内子的拼接中发挥着关键的核作用.
- 通过mago,EJC调节MAPK的表达,这对眼睛发育至关重要.
- 马戈的功能扩展到对异色染色体中的调节基因.
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