颗粒素细胞连接体NPPC及其受体NPR2在小鼠卵细胞中保持介质性停止
Meijia Zhang1, You-Qiang Su, Koji Sugiura
1State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, People's Republic of China.
Science (New York, N.Y.)
|October 16, 2010
概括
颗粒素细胞连接体尿素前体C型 (NPPC) 和其在积分细胞中的受体NPR2防止卵细胞中介性停止. 这种机制对于女性生育能力和及时排卵至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 细胞信号传递 细胞信号传递
- 发展生物学 发展生物学
背景情况:
- 哺乳动物卵细胞在 Graafian 毛囊中被 arrested 停留.
- 颗粒状细胞在维持这种介质性停止方面发挥着关键作用,防止过早成熟.
- 这种逮捕背后的分子机制尚未完全理解.
研究的目的:
- 调查尿素前体C型 (NPPC) 和其受体NPR2在维持卵细胞中介性停止中的作用.
- 为了阐明在颗粒细胞和累积细胞中涉及NPPC和NPR2的信号通路.
- 确定这种途径对女性生育能力和排卵同步的影响.
主要方法:
- 在小鼠壁画颗粒体和累积细胞中分析NPPC和NPR2mRNA表达.
- 在体外实验中测量cGMP水平和中介性恢复对NPPC的反应.
- 生成和分析Nppc和Npr2突变小鼠以评估体内功能.
主要成果:
- 壁状颗粒细胞表达NPPCmRNA,而积分细胞表达NPR2mRNA.
- NPPC治疗增加了累积细胞和卵细胞中的cGMP水平,在体外抑制了中介性恢复.
- Nppc和Npr2突变小鼠表现出受损的介质性停止,导致早期的介质性恢复.
- 发现卵细胞衍生因子可以促进细胞中的Npr2表达.
结论:
- NPPC-NPR2信号通路对于维持哺乳动物Graafian毛囊中的卵细胞中介性停止至关重要.
- 这一途径防止了早期的介质成熟,确保了适当的排卵时间和女性生育能力.
- 这个系统的失调会导致不孕症和发育异常.
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