一个与perylenediimide结合的DNA的疏水性自我组装成超分子聚合物
Prakash P Neelakandan1, Zhengzheng Pan, Mahesh Hariharan
1Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
Journal of the American Chemical Society
|October 20, 2010
概括
基因拖与疏水性链接器结合,自组合成线性和分支的超分子聚合物. 这种疏水性协会推动了基于DNA的材料的形成,为纳米技术提供了新的可能性.
科学领域:
- 超分子化学 超分子化学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- DNA纳米技术利用DNA的可编程基配对来构建复杂的结构.
- 疏水性相互作用在生物自我组装中至关重要,但在DNA结合体中未被探索.
- 二胺 (PDI) 链接剂为DNA结构引入疏水性质.
研究的目的:
- 为了研究DNA的自组装,使用水性PDI链接器对DNA的贝结合物进行了研究.
- 探索由疏水性协会驱动的超分子聚合物和聚合物的形成.
- 描述组装过程和由此产生的结构.
主要方法:
- 低温传输电子显微镜 (Cryo-TEM) 用于可视化组件.
- 紫外线和光谱学用于分析染色体含量.
- 盐跳停止流量测量用于动力分析.
- 原子力显微镜 (AFM) 用于高分辨率成像.
主要成果:
- 在稀释溶液中观察到10-30个DNA杆单体的线性端到端组合.
- 组装大小受子度,盐度和温度的影响.
- 动力分析揭示了一种由盐触发的同位体组装机制.
- 在较高度下观察到分支组件和组件捆绑.
结论:
- PDI链接器的疏水性联结驱动了DNA贝结合体的自我组装.
- 这项研究首次证明了对疏水性协会的使用,用于从DNA复合体中创建超分子聚合物.
- 这些发现为设计基于DNA的新材料和复杂聚合物打开了道路.
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