母亲Rnf12/RLIM是对小鼠印记X染色体失活的要求
Jongdae Shin1, Michael Bossenz, Young Chung
1Program in Gene Function and Expression, University of Massachusetts Medical School (UMMS), Worcester, Massachusetts 01605, USA.
Nature
|October 22, 2010
概括
母亲Rnf12/RLIM对于在雌性小鼠胚胎中启动印记X染色体失活 (XCI) 是必不可少的. 它的缺失破坏了XistRNA云的形成和父X染色体沉默,导致胚胎致死性.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在雌性哺乳动物中,X染色体失活 (XCI) 对于剂量补偿至关重要.
- 两个形式,印制和随机XCI,确保从性染色体适当的基因表达.
- 基因组RNA是关键的非编码RNA,用于沉默不活的X染色体.
研究的目的:
- 调查Rnf12/RLIM在印制XCI启动中的作用.
- 确定母乳Rnf12/RLIM对早期胚胎发育和XCI的影响.
主要方法:
- 在小鼠卵细胞中条件淘汰Rnf12.
- 在发育中的胚胎中分析XistRNA表达和X染色体沉默.
- 与缺乏Rnf12的胚胎干细胞进行比较.
主要成果:
- 突变Rnf12等位基因 (Δm) 的母体传播导致女性胚胎的死亡,原因是XCI印记有缺陷.
- Δm 雌性胚胎显示出抑制的Xist云形成和父X染色体沉默.
- 缺乏RLIM的胚胎干细胞仍然可以在随机XCI期间形成Xist云并使一些X链基因沉默.
结论:
- 孕产妇Rnf12/RLIM对于印制XCI的启动至关重要.
- 印制XCI中的RLIM的功能与随机XCI中的其作用不同.
- 这项研究强调了母亲因素在早期表观遗传编程中的重要性.
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