使用点击功能化的聚合物囊向癌细胞
Marloes M J Kamphuis1, Angus P R Johnston, Georgina K Such
1Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Victoria 3010, Australia.
Journal of the American Chemical Society
|October 28, 2010
概括
用抗体功能化的纳米工程聚合物囊精确地准癌细胞. 这种抗体功能化囊技术为向药物输送提供了一个有希望的方法,最大限度地减少副作用.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 药物运输 药物运输 药物运输
背景情况:
- 有针对性的药物输送旨在最大限度地提高目标部位的治疗疗效,同时最大限度地降低全身毒性.
- 纳米工程制造的药物载体,包括聚合物囊,为增强药物输送提供了多功能平台.
- 这些载体与准分子的功能化对于特定的细胞识别至关重要.
研究的目的:
- 开发一种用于使纳米工程聚合物囊具有抗体的通用方法.
- 为了评估抗体功能化囊对结直肠癌细胞的特定结合.
- 为了证明这种针对性传递系统在癌症治疗中的潜力.
主要方法:
- 利用点击化学来有效地功能化具有抗体的低污染,纳米工程聚合物囊.
- 同培养的抗体功能化囊与混合细胞群.
- 使用显微镜和细胞计数技术量化囊与结直肠癌细胞的特定结合.
主要成果:
- 通过点击化学成功使聚合物囊与抗体具有功能.
- 证明了抗体功能化囊对结直肠癌细胞的特定结合.
- 即使癌细胞占总细胞数量的不到0.1%时,也可以实现精确的向.
结论:
- 抗体功能化的聚合物囊为癌细胞提供了高度特定的向能力.
- 这种方法具有显著的前景,可以通过有针对性的输送来改善药物的治疗指数.
- 开发的方法为开发向纳米药物提供了一个多功能平台.
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