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由自组装的M(12) L(24) 分子球和互补的寡核酸结合模板的精确定义的DNA纳米粒子

Takashi Kikuchi¹, Sota Sato, Makoto Fujita

¹Department of Applied Chemistry, School of Engineering, The University of Tokyo, and JST-CREST, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Journal of the American Chemical Society

|October 29, 2010

概括

研究人员使用离子和DNA连接体创建了统一的DNA纳米粒子. 这些纳米粒子选择性地与特定的DNA序列聚合,使得有针对性的分子组装成为可能.

科学领域:

超分子化学超分子化学
纳米技术纳米技术
分子生物学分子生物学

背景情况:

自组装是创建复杂纳米结构的关键策略.
DNA纳米技术提供了对分子组织的精确控制.
的协调化学提供了纳米材料的多功能构建模块.

研究的目的:

为了合成完美单分散的DNA纳米粒子.
为了研究DNA纳米粒子与离子和连接体的自我组装.
探索这些纳米颗粒的选择性聚合与互补的DNA序列.

主要方法:

12个(II) 离子和24个双联体的自组合.
用不同长度的寡核酸对联体的功能化 (n=1-3).
研究纳米粒子与互补DNA (cDNA) 和核酸链的相互作用.

主要成果:

实现了完美单分散的DNA纳米粒子.
在DNA纳米粒子和cDNA链之间证明了键的形成.
观察到不溶性聚合物的形成完全与匹配的三元核酸链 (tri-A) 结合在一起.

结论:

合成的DNA纳米粒子表现出精确的自我组装特性.
这些纳米粒子可以通过键选择性地与特定的DNA序列结合.
这些发现支持开发基于DNA的纳米材料,用于有针对性的分子识别和组装.

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