相关实验视频

Updated: Jun 7, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

在DNA纳米结构中的G-四重复的动态构造切换的可视化

Yuta Sannohe¹, Masayuki Endo, Yousuke Katsuda

¹Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.

Journal of the American Chemical Society

|October 30, 2010

概括

研究人员使用DNA纳米支架实时观察G-四重复形成. 这种由离子诱导的四链DNA结构,通过原子力显微镜可视化改变形状.

科学领域:

分子生物学分子生物学
纳米技术纳米技术
生物物理学的生物物理.

背景情况:

G四复合体是具有重要的生物作用的四链核酸结构.
了解G-四形形成动态对于各种应用至关重要.
DNA纳米提供了一个研究纳米级分子相互作用的平台.

研究的目的:

实时观察G-四重复结构的形成和破坏.
为了研究由G-四重复形成引起的DNA复合体的构造变化.
为了利用DNA纳米支架可视化这些动态过程.

主要方法:

将富含G的链和DNA复合体纳入DNA纳米支架.
使用离子 (K(+)) 诱导G-四重复形成.
使用快速扫描原子力显微镜 (FS-AFM) 实时可视化结构变化.

主要成果:

在K(+) 存在的情况下,成功形成一个跨链 (3 + 1) G-四重复结构.
在去除K(+) 时,G-四重复结构的破坏已被证明.
实时观察DNA纳米支架中的全球形状变化,与G-四重复力学相关联.

结论:

这项研究提供了直接可视化G-四方体的形成和溶解.
DNA纳米是研究动态DNA结构的有效平台.
快速扫描原子力显微镜能够实时监测纳米级分子事件.

更多相关视频

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

相关概念视频

Single-Strand DNA Binding Proteins

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...