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相关概念视频

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

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相关实验视频

Updated: Jun 7, 2026

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
07:53

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Published on: January 1, 2018

植物和动物发育中的表观遗传重编程.

Suhua Feng1, Steven E Jacobsen, Wolf Reik

  • 1Howard Hughes Medical Institute and Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.

Science (New York, N.Y.)
|October 30, 2010
PubMed
概括

表观遗传重编程在生殖细胞和早期胚胎中重置基因组,涉及DNA脱甲基和基因素重塑. 这一过程对于物种间的发育,遗传和全能性至关重要.

科学领域:

  • 基因组学就是基因组学.
  • 发展生物学 发展生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 表观遗传修饰,如DNA甲基化和基因素标记,在体细胞中通常是稳定的.
  • 然而,显著的表观遗传重编程发生在生殖细胞和早期胚胎中.
  • 这种重编程涉及到全基因组的表观遗传标记的删除.

研究的目的:

  • 阐明全基因组表观遗传重编程的机制.
  • 了解表观遗传重编程在发育和遗传中的作用.
  • 为了比较不同生物体中的重编程策略.

主要方法:

  • 研究DNA脱甲基化途径,包括对5-甲基细胞素的修改.
  • 研究涉及表观遗传除的DNA修复机制.
  • 分析小RNA和基因素标记遗传中的作用.

主要成果:

  • 正在发现全基因组删除DNA甲基化机制.
  • 表观遗传重编程对于像印记和全能性获取等过程至关重要.
  • 小RNA和基因素标记可能在表观遗传和重编程中发挥作用.

结论:

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Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency
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Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency

Published on: February 2, 2024

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
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Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

  • 表观遗传重编程是繁殖和发育的一个基本过程.
  • 植物和哺乳动物之间的重编程的相似之处和差异突出了不同的策略.
  • 了解重编程是从发育生物学到跨代遗传等领域的关键.