一个尺寸值限制了子传输,并建立了表型多样性
Aaron Derdowski1, Suzanne S Sindi, Courtney L Klaips
1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 185 Meeting Street, Post Office Box G-L2, Providence, RI 02912, USA.
概括
蛋白形状影响聚合体大小,影响传播并导致年龄相关的表型变异. 这表明子构造指定表型作为动态群体平均值.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 子假设假设改变的蛋白质构造导致自我复制的聚合物,导致非典型的表型.
- 了解这些蛋白质错折路径如何与细胞环境相互作用以产生可传播的表型仍然是一个挑战.
研究的目的:
- 在细胞环境中研究蛋白形状,聚合特征和表型变异性之间的关系.
- 阐明了以为媒介的表型遗传和波动背后的机制.
主要方法:
- 使用酵母子模型系统 (Sup35/[PSI(+) ]) 来研究子蛋白聚合.
- 分析了蛋白质构成对总体尺寸分布的影响,通过与分子伴侣的相互作用.
- 研究了聚合丰度,传播值,生长和碎片化在表型变异中的作用.
主要成果:
- 发现蛋白质构造通过伴侣相互作用决定了普里昂聚合物的尺寸分布.
- 总体大小的变化导致了由于传输大小值,细胞之间的总体丰度差异.
- 总体动态 (生长,分裂) 和细胞间异质性导致了年龄相关的表型波动.
结论:
- 蛋白形状可以通过影响聚合动力学和细胞异质性来确定表型.
- 子系统中的表型结果最好被理解为动态,随机环境中的种群平均值.
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