超分子宿主-客组件的静电对接到通过双向光诱导电子转移探测的细胞染色体c
Katarzyna I Jankowska1, Cynthia V Pagba, Eugene L Piatnitski Chekler
1Department of Chemistry, Rutgers University, 73 Warren Street, Newark, New Jersey 07102, United States.
Journal of the American Chemical Society
|November 3, 2010
概括
一种新型的水溶性半体,作为一个航天飞机,向蛋白质提供氧化还原活性客体. 这种超分子组合增强了电子转移速率,模仿了天然蛋白质相互作用.
科学领域:
- 超分子化学 超分子化学
- 生物物理化学 生物物理化学
- 生物化学 生物化学
背景情况:
- 半监狱动物是多才多艺的宿主,能够封装客分子.
- 生物系统利用特定的相互作用进行分子识别和电子转移.
- 细胞染色体c是参与电子运输的关键蛋白质.
研究的目的:
- 为了研究水溶性八碳氧化化学的使用,并作为对氧化还原活性基质的航天器,以准蛋白质.
- 探索与生物分子的超分子组合中的多价值相互作用和形状灵活性的作用.
- 通过光诱导的电子转移,探究半癌客复合体和细胞染色体c之间的关联.
主要方法:
- 在半癌体内封装水电子捐赠者/接受者.
- 在Zn替代的cytochromec和hemicarceplexes之间进行光诱导电子转移 (ET) 的研究.
- 在不同的约束限值下,对三元蛋白质 - 化学癌症和客户组合的研究.
主要成果:
- 半癌有效地运输和传递氧化还原活性客体到细胞染色体c.
- 由蛋白质结合的半癌介导的ET显著比自由扩散快.
- 结合相互作用表明,半细胞复合体和细胞染色体c的活性部位之间存在表面互补性.
结论:
- 微弱的多价值相互作用和形状灵活性使得超分子组件与蛋白质的可逆结合成为可能.
- 半癌可以充当人工受体,通过靠近蛋白质活性部位来提高ET速率.
- 观察到的结合与自然的氧化还原合作伙伴相互作用一致,突出显示了生物模拟系统中半癌素的潜力.
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