骨髓衍生细胞有助于血管炎症,但不会分化为光滑肌肉细胞系
Hiroshi Iwata1, Ichiro Manabe, Katsuhito Fujiu
1Department of Cardiovascular Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Circulation
|November 3, 2010
概括
在受伤的动脉中表达光滑肌肉α-actin的骨髓衍生细胞是炎症单细胞,而不是光滑肌肉细胞. 这些细胞有助于血管重塑,但没有采用明确的光滑肌肉血统.
科学领域:
- 血管生物学 血管生物学
- 细胞谱系 细胞谱系
- 免疫学 免疫学 免疫学
背景情况:
- 假设来自骨髓的细胞透到新内中,并分化为光滑肌肉 (SM) 细胞.
- 之前的研究在确定这些骨髓衍生的精确血统方面遇到了技术上的限制.
研究的目的:
- 为了澄清骨髓衍生细胞在血管病变中表达光滑肌肉 (SM) α-actin的血统.
- 要确定这些细胞是否分化为最终的光滑肌肉细胞.
主要方法:
- 采用了针对SM-MHC的特定抗体和SM-MHC和SMα-actin的记者基因小鼠线.
- 分析了电线损伤诱导的neointima,在apolipoprotein E(-/-) 小鼠中的血管病变,以及心脏移植血管病变模型.
- 使用表面标记物 (CD115,CD11b,F4/80,Ly-6C) 和评估与炎症相关的基因表达的骨髓衍生细胞的表型.
主要成果:
- 骨髓衍生细胞表达SMα-actin在neointima没有表达明确的SM-MHC标志物,即使是长期的伤害后.
- 这些SMα-actin表达细胞表现出炎症单细胞的表型 (CD115(+) CD11b(+) F4/80(+) Ly-6C(+)).
- 通过收养转移的炎症单细胞在受伤的动脉中表达SMα-actin,并且这些细胞的区域内炎症基因表达升高.
结论:
- 骨髓衍生SMα-actin(+) 细胞主要是单细胞/巨细胞系.
- 这些细胞在血管重塑中发挥作用.
- 这些细胞极不可能获得明确的光滑肌肉细胞系.
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