相关实验视频

Updated: May 8, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

细菌免疫系统的CRISPR/Cas可以将菌体和等离子体DNA切割

Josiane E Garneau1, Marie-Ève Dupuis, Manuela Villion

  • 1Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Groupe de recherche en écologie buccale, Faculté de médecine dentaire, Félix d'Hérelle Reference Center for Bacterial Viruses, Université Laval, Quebec City, Quebec G1V 0A6, Canada.

Nature
|November 5, 2010
PubMed
概括

菌热球菌CRISPR1/Cas系统可以从等离子体中获得间隔器,导致等离子体损失并提供对抗生素耐药性基因的免疫力. 这种细菌免疫系统迅速分裂入侵的DNA.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System11:49

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System

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