人类红色球体转录因子的结构和演变
C D Trainor1, T Evans, G Felsenfeld
1Laboratory of Molecular Bikology, NIDDK, Bethesda, Maryland 20892.
Nature
|January 4, 1990
概括
研究人员克隆了人类Eryf 1基因cDNA,这对红细胞发育至关重要. 这个转录因子是转录因子.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 血液学 血液学 血液学
背景情况:
- 脊椎动物的红状腺细胞利用一种特定于组织的转录因子,称为Eryf 1,GF-1或NF-E1.1.
- 这种因子的结合点在全球蛋白基因家族和其他红色素特异性基因的调节区域中普遍存在.
- 人因子与突变部位的功能障碍结合与胎儿血红蛋白 (HPFH) 的遗传性持久性有关.
研究的目的:
- 报告人类Eryf 1编码基因的补充DNA (cDNA) 的克隆.
- 为了比较人类的Eryf 1基因序列与其和小鼠对应物.
主要方法:
- 人类Eryf 1cDNA的克隆.
- 人类,和老鼠Eryf 1cDNA的序列分析和比较.
主要成果:
- 人类Eryf 1cDNA中部三分之一,包含两个"手指"图案,与和小鼠版本具有很高的相似性.
- 人类蛋白质的氨基和碳氧末端区域与小鼠相似,但与有显著不同.
- 进化分析表明,Eryf 1因子从一个具有不同域的共同前体进化,以不同的速度分化.
结论:
- 人类Eryf 1的克隆为研究红状腺基因调节提供了关键工具.
- 对比分析揭示了脊椎动物中Eryf 1的保存和分离的进化路径.
- 了解Eryf 1的结构和演变,可以深入了解红状腺发育和HPFH等相关疾病.
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