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相关概念视频

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Cross-reactivity00:42

Cross-reactivity

Overview
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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相关实验视频

Updated: Jun 7, 2026

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

在基于S-RNase的自我不兼容性中的协作非自我识别系统.

Ken-ichi Kubo1, Tetsuyuki Entani, Akie Takara

  • 1Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.

Science (New York, N.Y.)
|November 6, 2010
PubMed
概括
此摘要是机器生成的。

开花植物利用自我不相容性来确保遗传多样性. 在Petunia中,多个S-locus F-box (SLF) 蛋白质协作识别多种非自我S-核核糖酶,从而实现兼容的授粉.

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Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
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Published on: June 16, 2020

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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相关实验视频

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Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

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Published on: June 16, 2020

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

科学领域:

  • 植物生殖生物学 植物生殖生物学
  • 分子遗传学 分子遗传学
  • 进化生物学是进化的生物学.

背景情况:

  • 自我不相容性 (SI) 是开花植物的一个关键机制,防止内生繁殖,通过外交促进遗传多样性.
  • 在Solanaceae家族中,S-locus F-box (SLF) 基因编码了花粉决定性,而S-核核糖酶 (S-RNases) 在SI中代表了结决定性.
  • 在SI研究中的一个关键问题是,鉴于SLF的较低的等位基多样性,花粉决定因 (SLF) 如何识别广泛的非自身决定因 (S-RNases).

研究的目的:

  • 研究SLF蛋白质识别Petunia中各种S-RNase的机制.
  • 阐明花粉决定因子在识别非自我S-RNases中的协作性.
  • 了解Solanaceae中自我不相容性的分子基础.

主要方法:

  • 在体内功能测试被用来测试SLF蛋白在花粉管中的活性.
  • 进行了蛋白相互作用测试,以确定SLF和S-RNase蛋白之间的结合特异性.
  • 进行了SLF和S-RNase等位基因的比较序列分析.

主要成果:

  • 在Petunia中,至少有三种不同类型的分离SLF蛋白被确定为功能性花粉决定因素.
  • 每种类型的SLF蛋白都表现出识别非自我S-RNases的特定子集的能力.
  • 这些发现表明一种协作识别系统,而不是SLF和S-RNase之间的一对一相互作用.

结论:

  • 这项研究揭示了Petunia的自我不兼容性中的多个SLF蛋白质介导的协作非自我识别系统.
  • 这种协作机制允许识别广泛的非自我S-RNase,解决SLF识别能力的之前难题.
  • 这些发现有助于更深入地了解植物自我不相容性和遗传多样性维护背后的分子机制.