酵母非编码RNA的进化揭示了广泛传播的内部损失的替代机制
Quinn M Mitrovich1, Brian B Tuch, Francisco M De La Vega
1Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143-2200, USA.
概括
酵母菌血统之间,内部损失机制有所不同. 在Candida albicans中,小核核RNA (snoRNA) 基因保留了内内的snoRNA,而Saccharomyces酵母通过拼接信号退化而失去内.
科学领域:
- 进化生物学是进化的生物学.
- 分子遗传学 分子遗传学
- 基因组学就是基因组学.
背景情况:
- 内子损失的进化驱动因素在很大程度上是未知的.
- 之前的研究主要研究了蛋白质编码基因中的内子损失.
- 小核RNA (snoRNA) 基因,其中内核通常是功能性的,提供了一个独特的模型系统.
研究的目的:
- 为了研究非编码的snoRNA基因中引子损失的进化机制.
- 为了比较不同酵母系,特别是Candida albicans和Saccharomyces的snoRNA基因中的内子损失模式.
- 阐明编码基因与非编码基因中的内子损失的独特进化轨迹.
主要方法:
- 深度RNA测序用于分析基因表达和识别拼接接口.
- 进行了全基因组的拼接接口注释,以映射内子-外子结构.
- 在不同的酵母系中进行了比较基因组分析.
主要成果:
- 在Candida albicans血统中,snoRNA基因表现出极端的紧缩和外子损失,但保留了含有功能性snoRNA的内子.
- 在Saccharomyces血统中,由于必要的拼接信号的退化,内部子被大量丢失.
- 在snoRNA基因中的这种内子损失在机制上和时间上不同于在蛋白质编码基因中观察到的.
结论:
- 与蛋白质编码基因相比,snoRNA基因中的内部损失遵循不同的进化途径.
- 在Saccharomyces的snoRNA基因中,内子的损失可能是由新型snoRNA处理机制促进的.
- 了解非编码RNA中的内子损失,为基因组进化提供了关键的见解.
相关概念视频
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Gene Evolution - Fast or Slow?
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...

