平面偏振的actomyosin收缩的流量控制上皮质结重塑
Matteo Rauzi1, Pierre-François Lenne, Thomas Lecuit
1IBDML, UMR6216 CNRS-Université de la Méditerranée, Campus de Luminy, case 907, 13288 Marseille Cedex 09, France.
表皮细胞的重塑依赖于向结点流动的肌肉蛋白II (一种actomyosin网络),而不仅仅是静态肌肉蛋白. 这种流动是由E-cadherin的分布引导的,驱动Drosophila胚胎中的组织延长.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 生物物理学的生物物理.
背景情况:
- 肌酶II电机产生了对细胞和组织形态发生至关重要的力量.
- 由E-cadherin增强的上皮结,抵抗收缩力并传递张力.
- 德洛索菲拉胚胎生殖带延伸涉及细胞间隔和平面偏振对上皮结的重塑.
研究的目的:
- 在上皮细胞变形中研究力生成和皮质力传递之间的相互作用.
- 澄清驱动Drosophila生殖带延伸期间背腔腹腔导向结合的收缩机制.
- 确定肌二分泌和动肌流在上皮细胞形态发生过程中的作用.
主要方法:
- 使用Drosophila melanogaster作为一个模型生物.
- 在胚胎生殖带延伸过程中研究了结节重塑和细胞变形.
- 分析了肌肉蛋白II,E-cadherin和α-Catenin在力传递和actomyosin动态中的作用.
主要成果:
- 交点收缩是由中位动素脉冲向背腹交点的两极流动驱动的,而不是由交点肌素II驱动的.
- 无向性actomyosin流由E-cadherin复合体的平面偏振分布来指导.
- 介质肌酸二流向有较少E-cadherin的结点流动,这表明通过α-Catenin传递力和E-cadherin合的平衡性质.
结论:
- 表皮形态发生是由两极化的收缩性actomyosin流调节的,而不仅仅是myosin II平稳态分布.
- E-cadherin和actomyosin网络之间的相互作用决定了中间流动模式.
- 这项研究揭示了一种新的力量传递和表皮质组织重塑的机制.
更多相关视频
12:35Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
Published on: April 14, 2023
08:50The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
Published on: March 10, 2023
相关概念视频
Cell Motility through Blebbing
Blebbing Through the Matrix
In multicellular...
Actin Polymerization and Cell Motility
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
The Role of Actin and Myosin in Non-muscle Cells
Role of Myosin in Cell Migration
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction. It is...
Mechanism of Lamellipodia Formation
Cytoskeletal Coordination in Cell Migration
