Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Precision therapeutic tRNA rescue of nonsense mutation R166X in <i>KCNJ13</i> to restore K<sup>+</sup> channel function.

Journal of precision medicine (Amsterdam, Netherlands)·2026
Same author

Focused CRISPR screening to design fit-for-purpose CAR T cell therapies.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Control of naive T cell reactivity and peripheral tolerance by ascorbate and TET activity.

Science advances·2026
Same author

Directing fratricide within T cell products using an anti-uPAR chimeric antigen receptor to drive the production of potent therapeutic cells.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Synonymous editing alters ion channel function, favoring prime editing for retinal disease correction.

International journal of biological sciences·2026
Same author

uPAR exhibits age- and region-dependent expression in the brains of mice with Alzheimer's disease-like pathology.

Brain research·2026

相关实验视频

Updated: Jun 6, 2026

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

多能性和细胞重编程:事实,假设,未解决的问题.

Jacob H Hanna1, Krishanu Saha, Rudolf Jaenisch

  • 1The Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. hanna@wi.mit.edu

Cell
|November 16, 2010
PubMed
概括
此摘要是机器生成的。

直接重编程产生诱导的多能干细胞,引发了关于表观遗传稳定性的问题. 了解分子和表观遗传因素对于实现体外重编程细胞的潜力至关重要.

更多相关视频

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency
07:08

Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency

Published on: February 2, 2024

相关实验视频

Last Updated: Jun 6, 2026

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
10:32

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Published on: September 6, 2014

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency
07:08

Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency

Published on: February 2, 2024

科学领域:

  • 细胞重新编程的细胞重编程.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 干细胞生物学 干细胞生物学

背景情况:

  • 将体细胞直接重编程为诱导多能干细胞 (iPSCs) 挑战了关于细胞身份的既定观点.
  • 众所周知,不同的多能状态会相互转换,受到内部和外部因素的影响.

研究的目的:

  • 审查了解细胞命运转换的分子和表观遗传决定因素的最新进展.
  • 突出细胞重编程领域未解决和有争议的问题.

主要方法:

  • 关于直接重编程和多能性的最近研究的文献综述.
  • 分析细胞状态转换背后的分子和表观遗传机制.

主要成果:

  • 转录因子的子宫外表达诱导多能性,但引发了关于表观遗传稳定性的问题.
  • 有证据表明,不同多能状态之间的相互转换是可能的.
  • 了解这些决定因素是利用体外重编程细胞的关键.

结论:

  • 需要进一步的研究才能充分阐明控制细胞重编程的分子和表观遗传环境.
  • 解决有争议的问题对于推进iPSCs的治疗应用至关重要.