相关实验视频

Updated: May 6, 2026

Principles of Site-Specific Recombinase SSR Technology

Principles of Site-Specific Recombinase SSR Technology

Published on: May 29, 2008

ROS的转录调节控制了从增殖到分化在根部的过渡

Hironaka Tsukagoshi¹, Wolfgang Busch, Philip N Benfey

¹Department of Biology and Institute for Genome Science and Policy Center for Systems Biology, Duke University, Durham, NC 27708, USA.

|November 16, 2010

概括

一种新发现的转录因子UPBEAT1 (UPB1) 通过调节活性氧物种 (ROS) 水平来控制植物的发育. 这一发现揭示了植物和动物细胞分化的保存机制.

科学领域:

植物生物学植物生物学
发展生物学发展生物学
细胞信号传输细胞信号传输

背景情况:

细胞增殖和分化是多细胞生物发育的基本过程.
保持这些过程之间的平衡对于适当的增长和发展至关重要.
在控制这种平衡方面,特定的监管因素的作用是一个活跃的研究领域.

研究的目的:

确定植物细胞增殖和分化之间的平衡的关键调节者.
阐明这些调节器运作的分子机制.
调查这些机制在不同王国的潜在保护.

主要方法:

在Arabidopsis根中的高分辨率表达数据分析.
全基因组表达特征. 全基因组表达特征.
染色体免疫沉与芯片分析 (ChIP-chip) 相结合.
反应性氧物种 (ROS) 和过氧酶活性的化学调制.

主要成果:

鉴定UPBEAT1 (UPB1) 作为调节增殖-分化平衡的转录因子.
UPB1直接控制过氧化酶基因表达,调节ROS水平.
由于ROS平衡的改变,UPB1的破坏导致分化延迟.
通过UPB1介导的途径独立于auxin和cytokinin信号传递.

结论:

UPBEAT1是Arabidopsis中细胞增殖和分化之间的平衡的关键调节者.
UPB1-氧化酶-ROS通路控制着分化开始的过程.
这种调节机制在植物和动物之间得到保护,突出了增长控制的共同策略.

更多相关视频

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

相关概念视频

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

Transcriptional Regulation: Riboswitches

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...