细菌核糖酶P全酶与tRNA复合体中的结构
Nicholas J Reiter1, Amy Osterman, Alfredo Torres-Larios
1Department of Molecular Biosciences, Northwestern University, Evanston, Illinois 60208, USA.
Nature
|November 16, 2010
概括
与tRNA结合的Ribonuclease P (RNase P) 全酶的晶体结构揭示了它的催化机制. 这项研究阐明了RNA-RNA相互作用和对于tRNA处理至关重要的活性位点.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 分子生物学分子生物学
背景情况:
- 核酶P (RNase P) 是一个重要的酶,负责tRNA5'-end成熟.
- RNase P是一种 ribozyme,这意味着它的催化活性主要是基于RNA的.
- 了解RNase P的结构是解读其催化机制的关键.
研究的目的:
- 确定与tRNA的复合体中的Thermotoga海洋RNase P全酶的高分辨率晶体结构.
- 阐明调控基质识别和催化物的分子相互作用.
- 提供对RNase P函数的通用机制的见解.
主要方法:
- 采用X射线晶体学,获得154kDa的RNase P-tRNA复合物的结构.
- 用前tRNA和金属离子进行浸泡实验,以确定基质结合和催化部位.
- 结构分析的重点是RNA-RNA相互作用,蛋白质-RNA接口和活性位点组成.
主要成果:
- 晶体结构透露了复杂的RNA-RNA识别,通过形状互补性,特定接触和基配对.
- 蛋白质辅因子与保存的RNA模块结合,并与前tRNA领导序列相互作用,而不是成熟的tRNA.
- 活性部位包括酸盐骨干元素,保存的尿素和必需的金属离子,表明保存的催化机制.
结论:
- 这项研究为RNase P介导的tRNA处理提供了详细的结构基础.
- 这些发现突显了RNA和蛋白质成分在基质结合和催化中的作用.
- 保存的特征表明,在不同的生物体中,RNase P具有普遍的催化机制.
相关概念视频
RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes can be...


