封装的能量传输盒带有非常好的光输出
Yuichiro Ueno1, Jiney Jose, Aurore Loudet
1Department of Chemistry, Texas A & M University, Box 30012, College Station, Texas 77841, USA.
Journal of the American Chemical Society
|November 26, 2010
概括
研究人员使用BODIPY和氨酸染料开发了新的与水相容的光成像探针. 这些探头被纳米颗粒封装,可以通过可调节的单波长激发和红色发射,在线粒体中定位,实现高效的细胞成像.
科学领域:
- 化学生物学 化学生物学
- 材料科学 材料科学 材料科学
- 生物物理学的生物物理.
背景情况:
- 光成像探针的开发对生物研究至关重要.
- 现有的探测器往往缺乏可调节的光子特性,或需要多个激发波长.
- 需要具有大型光谱窗口的水兼容探头来进行多重复合.
研究的目的:
- 开发具有可调节光子性能的水兼容光成像探头.
- 为了研究双色光带中的能量传递机制.
- 为了使细胞成像和器官标记能够使用单一的激发源.
主要方法:
- 合成双染色体录音带 (BODIPY供体,素接受体).
- 光物理特征包括激发,排放和能量转移速率测量.
- 将探针封装成酸/酸盐纳米颗粒.
- 在大鼠肝细胞中进行细胞吸收和局部化研究.
主要成果:
- 双色光带表现出高效的通过结合的能量传输,使得大型准斯托克斯转移 (86290 nm).
- 探测器在单波长激发时显示可调节的发射在600,700和800纳米.
- 纳米粒子封装提高了水的分散性,对光物理性质的影响最小.
- 封装式探针成功进入细胞并定位在线粒体中,发射红色光.
结论:
- 易于访问的酸基磁带为先进的成像提供了高效的透过键的能量传输.
- 纳米粒子配方为生物应用提供了水的兼容性.
- 这些探针适用于活细胞中的多重和有机体特异性标记.
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