相关实验视频
Updated: Jun 6, 2026

17:14
In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
与基质的APC/C(Cdh1) 的结构将Cdh1和Apc10确定为D盒共受体
Paula C A da Fonseca1, Eric H Kong, Ziguo Zhang
1Section of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London SW3 6JB, UK.
Nature
|November 26, 2010
概括
研究人员使用冷电子显微镜揭示了酶促进复合体 (APC/C) 如何通过破坏动机识别目标蛋白质. 这项研究澄清了APC/C基质无处不在的结构基础,这对于细胞周期控制至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 亚纳酶促进复合体 (APC/C) 通过使关键蛋白质无处不在来调节细胞分裂.
- APC/C的目标识别取决于像D-box这样的破坏动机.
- 在APC/C基质结合和无处不在的结构机制仍然不清楚.
研究的目的:
- 阐明APC/C.对基质识别的结构基础.
- 研究共激活蛋白 (Cdc20,Cdh1) 和核心APC/C子单元在标选择中的作用.
- 了解D-box识别所涉及的结构相互作用.
主要方法:
- 单粒子电子显微镜 (cryo-EM) 用于确定APC/C(Cdh1) 复合物的结构.
- 核磁共振 (NMR) 谱学用于分析D-box-Apc10相互作用.
- 差异映射用于识别APC/C复合体内的组件.
主要成果:
- 一个芽酵母APC/C(Cdh1) 与D-box结合的冷-EM图在~10 Å分辨率下确定.
- 在APC/C中央腔中发现了一个催化和基质识别模块,包括Cdh1,Apc10和Apc2.
- 通过NMR证实了特定的D-box-Apc10相互作用,突出显示了Apc10在D-box识别中的作用.
结论:
- 该研究为APC/C基质识别和催化提供了一个结构框架.
- 它澄清了协同激活器 (Cdh1) 和核心APC/C子单元 (Apc10) 在D-box识别中的联合作用.
- 这项工作促进了对通过APC/C调解的细胞循环控制机制的理解.
相关概念视频
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
Structure of Cadherins
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Clathrin Coated Vesicles
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

