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相关概念视频

Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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相关实验视频

Updated: Jun 6, 2026

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
07:53

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Published on: January 1, 2018

通过对内源蛋白反应的RNA控制器重新编程细胞行为.

Stephanie J Culler1, Kevin G Hoff, Christina D Smolke

  • 1Division of Chemistry and Chemical Engineering, 1200 East California Boulevard, MC 210-41, California Institute of Technology, Pasadena, CA 91125, USA.

Science (New York, N.Y.)
|November 27, 2010
PubMed
概括
此摘要是机器生成的。

研究人员设计了新的RNA控制装置,通过准替代RNA拼接来精确调节基因表达. 这一突破为细胞控制和重编程提供了新的合成生物学应用.

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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相关实验视频

Last Updated: Jun 6, 2026

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
07:53

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Published on: January 1, 2018

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

科学领域:

  • 合成生物学 合成生物学
  • 分子和细胞生物学分子和细胞生物学
  • 基因工程是一种基因工程.

背景情况:

  • 合成基因网络的工程具有挑战性,因为它们与原生细胞组件的接口存在困难.
  • 控制细胞行为需要精确的遗传装置,可以与内源途径相互作用.

研究的目的:

  • 开发一种新型的RNA控制装置,克服与原生细胞通路的接口限制.
  • 设计能够检测特定信号通路和重新编程细胞行为的设备.

主要方法:

  • 设计的RNA控制装置,通过替代RNA剪接调节将蛋白质丰度与基因表达结合起来.
  • 通过人类细胞中的核因子kappa B (NF-κB) 和Wnt信号通路检测信号的工程设备.

主要成果:

  • 证明了NF-κB和Wnt信号通路的成功重新连接,以诱导新的细胞行为.
  • 将疾病标志物与非侵入性传感联系起来,并使用工程RNA设备重新编程细胞命运.

结论:

  • 开发了一个多功能基因平台,用于创建可编程传感激活设备.
  • 通过将合成设备与本地途径接口,使细胞行为能够自主控制.