通过基因型特定的修饰剂对转基因表达和印记进行表观遗传控制
N D Allen1, M L Norris, M A Surani
1Department of Molecular Embryology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, England.
Cell
|June 1, 1990
概括
基因型特异性修饰基因控制转基因表达和DNA甲基化. 在TKZ751位点的表观遗传修饰可以在小鼠中变得不可逆转,影响基因透和印记.
科学领域:
- 遗传学 是一个遗传学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 调节转基因表达和DNA甲基化对于理解基因控制至关重要.
- 遗传背景可以影响表观遗传修饰和基因表达模式.
研究的目的:
- 研究基因型特异性修饰基因在控制TKZ751转基因位点的表达和DNA甲基化中的作用.
- 确定不同遗传背景 (DBA/2,129,BALB/c) 对转基因表观遗传修饰的影响.
- 检查跨代表观遗传修饰的遗传模式和可逆性.
主要方法:
- 分析不同小鼠遗传背景的转基因位点TKZ751表达和DNA甲基化.
- 研究BALB/c遗传背景对母亲遗传的影响.
- 在相继的几代人中追踪表观遗传修饰.
主要成果:
- DBA/2和129个基因背景增强了TKZ751的表达.
- BALB/c背景抑制表达,特别是与母亲的遗传.
- 表观遗传修饰是累积的,在BALB/c小鼠中,经过三次生殖线穿越后,变得不可逆转.
- 该生殖系未能在TKZ751位点逆转先前获得的表观遗传修饰.
结论:
- 基因型特定的修饰基因调节TKZ751位点的透性和表达性.
- 表观遗传修饰,包括DNA甲基化,在这种调节中起着关键作用.
- 该研究强调了在特定遗传环境中的TKZ751位点发生生殖系表观遗传重编程失败,影响了父母的印记.
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