相关实验视频
Updated: Jun 6, 2026
11:09
Chromatin Isolation by RNA Purification (ChIRP)
Published on: March 25, 2012
通过长长的内在非编码RNA进行Vernalization介导的表观遗传沉默
1Section of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, the University of Texas at Austin, TX 78712, USA.
Science (New York, N.Y.)
|December 4, 2010
概括
文化利用寒冷来表观遗传地使花C (FLC) 基因沉默. 一种新的长内基非编码RNA,COLDAIR,通过准PRC2到FLC,对这种冷诱导的表观遗传抑制至关重要.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 绿化是一个至关重要的过程,使得植物在冬季暴露于寒冷后,能够在春季开花.
- 这涉及到表观遗传沉默的花抑制剂,特别是花C (FLC) 在Arabidopsis.
- 聚合体抑制复合体2 (PRC2) 调解了这种由寒冷引起的表观遗传沉默.
研究的目的:
- 研究非编码RNAs在FLC的本地化媒介表观遗传沉默中的作用.
- 为了确定在冷暴露期间将PRC2准到FLC位点的新型因素.
主要方法:
- 在经过冷处理的阿拉比多普西斯植物中分析基因表达和染色质修饰.
- RNA免疫沉降 (RIP) 和染色体免疫沉降 (ChIP) 测试用于检测RNA-蛋白和蛋白质-DNA相互作用.
- 使用缺乏特定非编码RNA的突变物进行遗传分析.
主要成果:
- 一种名为COLDAIR (COLD ASSISTED INTRONIC NONCODING RNA) 的长内基非编码RNA被确定为本土化中的关键.
- COLDAIR与PRC2.2的一个子单元发生物理相互作用.
- 为了将PRC2向FLC位点,需要COLDAIR,从而导致稳定的表观遗传抑制.
结论:
- 寒冷是本土化途径的关键组成部分,作为PRC2向FLC基因的指南.
- 这种相互作用在FLC中建立了稳定的抑制色素,确保了应对冬季寒冷的适当开花时间.
- 科尔达尔代表了一种用于植物环境诱导的表观遗传调节的新机制.
相关概念视频
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
03:17Inheritance of Chromatin Structures
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...