来自Xenopus laevis细胞系的活性类因子,负责介质体诱导
A J van den Eijnden-Van Raaij1, E J van Zoelent, K van Nimmen
1Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Nature
|June 21, 1990
概括
使用生长因子,可以诱导Xenopus laevis ectoderm explants形成中皮层. 研究人员确定XTC细胞中的Xenopus laevis中皮诱导因子 (MIF) 是哺乳动物活性蛋白的同类物.
科学领域:
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 两动物胚胎发生过程中介质体的诱导对于发育至关重要.
- 像转化生长因子-β (TGF-β) 这样的生长因子可以在体外模仿这个过程.
- 来自Xenopus laevis XTC细胞的特定间皮诱导因子 (MIF) 仍然未被确定,尽管显示TGF-β类属性.
研究的目的:
- 研究由Xenopus laevis XTC细胞产生的中皮诱导因子 (MIF) 的性质.
- 分析MIF活性与XTC细胞克隆中的活性因活性之间的关系.
- 要确定XTC-MIF是否是哺乳动物活性蛋白的Xenopus同类物.
主要方法:
- 从异质的XTC细胞群中分离出同质细胞系.
- 检测不同XTC克隆的中皮诱导因子 (MIF) 生产.
- 在细胞提取物中测量转化生长因子-β (TGF-β) 活性和活性类活性.
- 在XTC细胞系中比较MIF活性与激素活性.
主要成果:
- 只有一个XTC克隆,XTC-GTX-11,产生了显著的MIF活动.
- 与其他克隆相比,这个克隆表现出类似的已知生长因子的产生,包括TGF-β活性.
- 在XTC细胞及其克隆细胞中,MIF活性和活性因子活性存在的明显和一致的相关性被观察到.
- 活性蛋白A被确定为一种强大的中皮诱导因子.
结论:
- 来自Xenopus laevis XTC细胞的间皮诱导因子 (MIF) 与激素活性密切相关.
- XTC-MIF被确定为哺乳动物活性蛋白的Xenopus同类物.
- 这一发现澄清了Xenopus laevis胚胎生成中介质体诱导的分子基础.
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