MYPT1-PP1互动的结构签名
Anderson S Pinheiro1, Joseph A Marsh, Julie D Forman-Kay
1Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, Rhode Island 02903, USA.
Journal of the American Chemical Society
|December 15, 2010
概括
用NMR研究了氨酸酸酶向子单元1 (MYPT1) 结构的未结合状态. 在MYPT1中,一个短暂的α螺旋是与蛋白质酸酶1 (PP1) 形成肌酸酸酶全酶复合物的关键.
科学领域:
- 结构生物学 结构生物学
- 生物化学 生物化学
- 分子动力学分子动力学
背景情况:
- 肌肉放松取决于肌酸酸酶 (MP) 的全酶活性.
- MYPT1将蛋白质酸酶1 (PP1) 向肌,并指导特异性.
- 了解MYPT1-PP1复合体形成需要了解MYPT1的无约束结构.
研究的目的:
- 确定未结合的MYPT1.1的结构和动态特性.
- 将MYPT1的无结合性构造与其PP1结合状态进行比较.
- 阐明推动MYPT1-PP1全酶形成的分子事件.
主要方法:
- 核磁共振 (NMR) 谱学分析未结合的MYPT1 ((1-98).
- 整合NMR和生物物理数据.
- 为MYPT1 ((1-98) 计算一个集合模型.
主要成果:
- 未结合的MYPT1 ((1-98) 呈现出两种域结构:一个动态无序区域 (残留1-40) 和一个刚性基林重复区域 (残留41-98).
- 一个短暂的α螺旋 (25%的人口) 存在于未结合的MYPT1 ((1-98) 的混乱区域.
- 与其他PP1调节器相比,MYPT1表现出独特的结构和动态行为.
结论:
- 在PP1结合后,MYPT1中的过渡性α螺旋变得完全填充,在全酶形成中起着至关重要的作用.
- 为MYPT1-PP1全酶组合提出了一个新型模型.
- 揭示了非绑定PP1调节器的独特结构和动态特性.
相关概念视频
Protein-protein Interfaces
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Phosphoinositides and PIPs
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...


