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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
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Published on: January 1, 2018

直接转化C. elegans生殖细胞成为特定类型的神经元.

Baris Tursun1, Tulsi Patel, Paschalis Kratsios

  • 1Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, NY 10032, USA. bt2189@columbia.edu

Science (New York, N.Y.)
|December 15, 2010
PubMed
概括

科学家通过转录因子将Caenorhabditis elegans生殖细胞重新编程成特定的神经元类型. 这种细胞身份转换需要去除基因素陪伴物LIN-53,突出染色素.

科学领域:

  • 发展生物学 发展生物学
  • 细胞重编程 细胞重编程
  • 神经科学是一个神经科学.

背景情况:

  • 细胞身份通常是固定和上下文依赖的,限制了直接的细胞重编程.
  • 转录因子是细胞命运的关键调节者,但它们的重编程能力通常受到限制.

研究的目的:

  • 研究Caenorhabditis elegans生殖细胞直接重新编程成特定的神经元亚型.
  • 确定促进或抑制这种生殖细胞转化为神经元的因素.

主要方法:

  • 单个Caenorhabditis elegans转录因子在生殖细胞中的异位表达.
  • 使用特定的神经元标记物的细胞身份转换的分析.
  • 在重编程过程中研究了基因素陪伴物LIN-53的作用.
  • 利用化学抑制组织素脱乙酶来模仿LIN-53的去除.

主要成果:

  • 成功地将线粒性生殖细胞转化为谷氨酸性,胆固醇性或GABAergic神经元类型.
  • 重编程效率取决于去除基因素陪伴物LIN-53.
  • 抑制基因素脱乙酶模仿了去除LIN-53的效果,促进了重编程.

结论:

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  • 胚胎细胞可以直接被重新编程成不同的,终端分化的神经元类型.
  • 基因素陪伴物LIN-53作为细胞重编程的障碍物.
  • 针对LIN-53等染色质因子可能为细胞命运转换提供新的策略.