通过基因组比较揭示了污垢真菌中的致病性决定因素
Jan Schirawski1, Gertrud Mannhaupt, Karin Münch
1Department of Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Straße 10, 35043 Marburg, Germany.
Science (New York, N.Y.)
|December 15, 2010
概括
玉米病原体Sporisorium reilianum和Ustilago maydis的比较基因组学确定了43个可变的基因组区域. 四个新的区域被证实是U. maydis的毒性决定因素,有助于发现病原体效应因子.
科学领域:
- 植物病理学 植物病理学
- 菌类基因组学 菌类基因组学
- 分子植物微生物相互作用.
背景情况:
- 生物性真菌病原体如Ustilago maydis和Sporisorium reilianum分泌蛋白质效应物来操纵宿主植物.
- 这些分泌的效应因子的快速演变对于病原体的毒性至关重要.
- 识别新的毒性因素是理解宿主-病原体动态的关键.
研究的目的:
- 通过比较基因组学识别S. reilianum中编码分泌效应物的可变基因组区域.
- 在U. maydis.中功能性地表征新的毒性决定因素.
主要方法:
- 在Sporisorium reilianum的基因组测序.
- 在S. reilianum和Ustilago maydis.之间进行比较基因组分析.
- 识别具有低序列保存的合成区域.
- 在U. maydis中进行删除分析,以评估已识别区域的毒性.
主要成果:
- 在S. reilianum和U. maydis基因组之间确定了43个低序列保存区域.
- 这些可变区域被丰富为编码分泌效应因子和已知的毒性集群的基因.
- 在U.中删除四个以前未被描述的多样性区域可能会对病毒毒性产生重大影响.
结论:
- 密切相关的真菌物种的比较基因组学是一种强大的方法来发现毒性决定因素.
- 居住在可变基因组区域的新型效应基因在真菌病原体毒性中起着关键作用.
- 了解这些效应因子-宿主相互作用可以为抗击植物疾病的策略提供信息.
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