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使用基因编码的NOR门和化学"线"进行强大的多细胞计算.

Alvin Tamsir¹, Jeffrey J Tabor, Christopher A Voigt

¹Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA.

|December 15, 2010

概括

科学家们设计了细菌以使用遗传电路和细胞间通信来执行复杂的计算. 这种合成生物学方法能够实现强大的逻辑门,为新的生物计算应用铺平了道路.

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科学领域:

合成生物学合成生物学
计算生物学计算生物学
微生物工程微生物工程

背景情况:

细胞组织和发育依赖于计算和细胞间通信.
生物膜中的细菌表现出从简单的个体操作中出现的模式.

研究的目的:

在大肠杆菌中设计一种能够进行复杂空间计算的基因电路.
为了利用定数感应作为生物逻辑门的通信机制.

主要方法:

在大肠杆菌中使用协同促进器和抑制器系统构建了一个NOR逻辑门.
集成的直角定数感应发送器和接收器设备连接逻辑门.
在空间上安排细菌殖民地,以创建多样化的计算功能.

主要成果:

成功生成了所有可能的双输入逻辑门,包括 XOR 和 EQUALS 函数.
证明了强有力的反应,在各州之间变化为5至300倍.
质量检测分子有效地作为工程门之间的通信线.

结论:

简单的遗传逻辑门可以组合在一起,在空间中执行复杂的计算.
重新连接细胞-细胞通信是设计各种生物计算的可行策略.
这项工作提供了在合成生物学中利用细胞计算的设计原则.

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