在B. subtilis的发育过程中对母细胞基因表达进行检查
1Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.
Cell
|July 27, 1990
概括
在B. subtilis中bofa和bofB基因的突变将母细胞基因表达与异位信号分离. 这一发现揭示了一个检查点,用于协调在发育中的体区间之间的基因表达.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细菌遗传学 细菌遗传学
背景情况:
- 在Bacillus subtilis球菌中,基因表达受到西格玛因子的调节,包括母细胞中的西格玛K.
- 矛盾的是,SIGMA K-依赖基因表达需要更多的调节蛋白,比如SIGMA G.
- 与母细胞基因表达联系的确切机制尚不清楚.
研究的目的:
- 为了确定基因因素介导母细胞基因表达的依赖性对异位调节蛋白.
- 阐明 bofA 和 bofB 基因在调节 sigma K 激活中的作用.
- 了解分泌过程中细菌细胞区间之间协调基因表达的检查点机制.
主要方法:
- 在bofA和bofB基因中的突变的识别和表征.
- 对野生型和突变B. subtilis菌株的基因表达模式的分析.
- 对pro-sigma K处理和激活的研究.
主要成果:
- 在bofA和bofB的突变减轻了母细胞基因表达的依赖性对spspore调节蛋白,但不是对sigma K.
- 这种依赖是通过K转换到其活性形式的pro-sigma K转换的水平来实现的.
- 似乎BofA和/或BofB蛋白调节这种转换,以响应一个异常生成的信号.
结论:
- BofA和BofB蛋白质作为关键调节剂,将异位信号与母细胞基因表达联系起来.
- 在化过程中,Pro-sigma K激活是分组间基因表达协调的关键检查点.
- 这些发现为管理Bacillus subtilis发育的复杂监管网络提供了新的见解.
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