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为T细胞刺激所需的抗原呈现细胞MHCII类/复合物的定量

C V Harding¹, E R Unanue

¹Department of Pathology, Washington University School of Medicine, St Louis, Missouri 63110.

|August 9, 1990

概括

估计T细胞激活所需的特定/MHC复合体的数量显示,每种抗原呈现细胞 (APC) 中只有210-340个复合体可以触发反应,解释了多抗原呈现.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

对于T细胞激活所需的抗原呈现细胞 (APC) 上的/MHCII类复合物的确切数量仍然未知.
外来的APC呈现发生在MHCII类分子可能已经被自我所占据时.

研究的目的:

估计每个APC需要激活T细胞所需的特定/MHCII类复合物的数量.
为了研究这个数字对同时异性抗原呈现的含义.

主要方法:

在用放射性标记的免疫原性质脉冲APC后,I-Ak分子的定量免疫沉.
通过呈现不同数量的/MHC复合体的APC激活T细胞.
确认使用抗CD3抗体涂层乳珠作为替代T细胞刺激剂.

主要成果:

每个APC中只有210-340个特定/MHCII类复合体足以激活T细胞.
这代表了APC表面上I-Ak分子总数的一小部分 (0.1%).
使用抗CD3抗体涂层珠子证实了低激活值.

结论:

令人惊的是,需要少量的特定/MHCII类复合体来激活T细胞.
这种低值解释了APC如何能够同时呈现多个外来抗原,即使是与竞争的自我.

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