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Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
Published on: October 6, 2011
抑制的空间不对称重组建立了一个运动敏感电路
Keisuke Yonehara1, Kamill Balint, Masaharu Noda
1Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
Nature
|December 21, 2010
概括
神经电路的发展建立空间不对称性在抑制输入方向选择性细胞. 这种快速的发育转换发生在两天内,塑造了神经元的处理.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 计算神经科学是一种神经科学.
背景情况:
- 神经连接表现出神经处理至关重要的空间不对称性.
- 定向选择性 (DS) 视网膜质细胞依赖于来自星爆亚马克林细胞的不对称抑制输入.
- 建立这种电路不对称性的发育机制在很大程度上是未知的.
研究的目的:
- 研究星爆-DS细胞连接中空间不对称性建立的发展时间表和机制.
- 了解如何以及何时在出生后早期发育过程中出现抑制性输入不对称.
主要方法:
- 通过光刺激道罗多普辛-2向小鼠星爆细胞.
- 从基因标记的DS细胞进行记录,以映射突触强度分布.
- 单突触限制的跨突触狂犬病病毒追踪以确认连接性.
主要成果:
- 星爆-DS细胞抑制输入不对称性在2天内迅速发展.
- 具有随机或对称连接的中间状态先于不对称.
- 发育涉及阻碍性突触输入的空间选择性重组.
- 来自星爆细胞的刺激输入比抑制输入更对称.
结论:
- 在主细胞电路中,表现出从对称到不对称的抑制输入分布的快速发展切换.
- 突出了神经发育期间突触输入组织的动态性质.
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