小鼠蛋白质酸化和表达的组织特异地图
Edward L Huttlin1, Mark P Jedrychowski, Joshua E Elias
1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Cell
|December 25, 2010
概括
这项研究表明,虽然大多数蛋白质是普遍存在的,但它们的酸化模式往往是特定于组织的. 这种酸化微调蛋白活性,优化每个组织独特的生理功能.
科学领域:
- 蛋白质组学和分子生物学
- 细胞信号传输和翻译后的修改
背景情况:
- 生物体具有基因相同的细胞,但组织表现出不同的生化专业化.
- 组织特异生理学依赖于调节的基因/蛋白质表达和细胞内信号传递,特别是酸化.
- 了解酸化的作用对于破译组织专业化和疾病至关重要.
研究的目的:
- 研究酸化在维持独特的组织生理学中的作用.
- 在多个小鼠组织中描述蛋白质和蛋白质的特征.
主要方法:
- 在九种不同的小鼠组织上进行了全面的蛋白质和蛋白质分析.
- 鉴定和量化了数千种蛋白质及其酸化位点.
主要成果:
- 鉴定了12,039种蛋白质,其中包括6,296种具有近36,000个酸化位的脂蛋白.
- 揭示了每个组织独特的专门的,相互连接的酸化网络.
- 证明了许多蛋白质是通过酸化调节的,独立于它们的表达水平.
结论:
- 酸化在特定组织的适应和功能中起着关键作用.
- 广泛表达的脂蛋白表现出可变的,组织特异的酸化,针对特定的生理需求调活动.
- 生成的数据集是生物研究的宝贵资源.
相关概念视频
02:54Protein Kinases and Phosphatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
02:54Protein Kinases and Phosphatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
01:02Phosphorylation
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...