在Bacillus subtilis菌体SPO1的DNA聚合酶基因中,一个自我拼接的I组内子
H Goodrich-Blair1, V Scarlato, J M Gott
1Department of Biological Sciences, State University of New York, Albany 12222.
Cell
|October 19, 1990
概括
研究人员在感染Bacillus subtilis的菌体SPO1中发现了一种自我拼接的内子. 这一发现为细菌中自剪接内子的早期进化起源提供了证据.
科学领域:
- 分子生物学分子生物学
- 进化生物学 进化生物学
- 病毒学 病毒学
背景情况:
- 自剪接的内子是能够从RNA转录中删除自身的遗传元素.
- 组IA内子是发现在真核细胞有机体和菌体T4中的一个特征很好的类别.
- 菌体 SPO1 感染了格拉姆阳性细菌 Bacillus subtilis.
研究的目的:
- 为了研究细菌SPO1.1.中的内子的存在和特征.
- 为了比较SPO1内与已知的IA组内.
- 探索细菌病毒中自我拼接内子的进化含义.
主要方法:
- 细菌SPO1基因组的序列分析.
- 内部的二次结构预测.
- 保存序列和结构图案的识别.
主要成果:
- 在菌体SPO1.1中发现了一种自我拼接的IA组内.
- 该SPO1内与真核生物和菌体T4组IA内共享保存特征.
- 内部包含一个开放的读取框架,编码类似于大肠杆菌DNA聚合酶I的DNA聚合酶.
结论:
- 病毒中存在自剪接内子,它们感染了阳性和阴性细菌.
- 这一发现支持生命进化的早期起源.
- SPO1内为研究内演变和功能提供了一个新的模型.
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