对蛋白质的CORM-3反应性:一个Ru(II) 二碳烯-酶复合物的晶体结构
Teresa Santos-Silva1, Abhik Mukhopadhyay, João D Seixas
1REQUIMTE-CQFB, Departamento de Química, FCT-UNL, 2829-516 Caparica, Portugal.
Journal of the American Chemical Society
|January 6, 2011
概括
CORM-3释放一氧化碳并与蛋白质结合,形成稳定的添加物. 这项研究揭示了CORM-3蛋白结合所涉及的特定分子相互作用和协调部位.
科学领域:
- 生物有机化学 生物有机化学
- 金属蛋白化学 金属蛋白化学
- 药物发现 药物发现 药物发现
背景情况:
- 一氧化碳释放分子 (CORM) 用于控制生物系统中的一氧化碳 (CO).
- CORM-3,[fac-Ru ((CO) ((3) Cl ((κ ((2) -H ((2) NCH ((2) CO ((2))) ],是已知的CORM,用于在体内输送CO.
研究的目的:
- 阐明CORM-3与蛋白质之间的分子相互作用.
- 描述由此产生的蛋白添加物及其协调化学.
主要方法:
- 感应合的等离子体-原子发射光谱 (ICP-AES)
- 液体染色体质谱法 (LC-MS) 是一种流体染色体质谱法.
- 红外光谱学 (IR) 红外光谱学
- 在X射线晶体学.
主要成果:
- 在与蛋白质相互作用时,CORM-3解离,失去化物,甘氨酸和一个CO联结体.
- 稳定的添加物形成在蛋白质和剩余的cis-Ru(II) ((CO) ((2) 片段之间.
- X射线结晶学在蛋白溶酶中发现了三个协调位,主要位点显示了fac-[{His15) Ru{CO}{2}{H}{2}{O}{3}}]结构,占用率为80%.
结论:
- 该研究提供了CORM-3蛋白相互作用的第一个详细的结构和化学特征.
- 了解这些引证对于CORM的治疗应用和设计新的金属药物至关重要.
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