Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Multivalent weak contacts shape chaperone-nascent protein interactions.

bioRxiv : the preprint server for biology·2026
Same author

Ensemble molecular mimicry correlates with antibody cross-reactivity in proteome-wide studies.

Frontiers in immunology·2026
Same author

Arrest Peptide Profiling resolves co-translational folding pathways and chaperone interactions in vivo.

Nature communications·2025
Same author

Chaperone saturation mediates translation and protein folding efficiency.

bioRxiv : the preprint server for biology·2025
Same author

The Ensemble Basis of Allostery and Function: Insights from Models of Local Unfolding.

Journal of molecular biology·2025
Same author

Impact of local unfolding fluctuations on the evolution of regional sequence preferences in proteins.

Protein science : a publication of the Protein Society·2025

相关实验视频

Updated: Jun 5, 2026

A Modular Workflow for Quantitative, Structural and Functional Analysis of Leptospira Biofilms
08:51

A Modular Workflow for Quantitative, Structural and Functional Analysis of Leptospira Biofilms

Published on: December 19, 2025

结构生物学:寻找湿点的方法

Vincent J Hilser

    Nature
    |January 14, 2011
    PubMed
    概括

    No abstract available in PubMed .

    更多相关视频

    In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
    07:42

    In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

    Published on: March 19, 2010

    In situ TEM of Biological Assemblies in Liquid
    08:28

    In situ TEM of Biological Assemblies in Liquid

    Published on: December 30, 2013

    相关实验视频

    Last Updated: Jun 5, 2026

    A Modular Workflow for Quantitative, Structural and Functional Analysis of Leptospira Biofilms
    08:51

    A Modular Workflow for Quantitative, Structural and Functional Analysis of Leptospira Biofilms

    Published on: December 19, 2025

    In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
    07:42

    In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

    Published on: March 19, 2010

    In situ TEM of Biological Assemblies in Liquid
    08:28

    In situ TEM of Biological Assemblies in Liquid

    Published on: December 30, 2013