凯诺哈比迪斯 elegans ras基因let-60在阴道诱导途径中充当开关
G J Beitel1, S G Clark, H R Horvitz
1Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Nature
|December 6, 1990
概括
在C. elegans中,let-60基因通过作为信号开关来控制阴道发育. 突变揭示了ras蛋白激活和功能在这个发育途径中的关键区域.
科学领域:
- 发育生物学 发展生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 莱特-60基因是 *Caenorhabditis elegans * 中的一个必不可少的ras基因.
- 它作为控制阴道形成的感应信号通路中的关键开关起作用.
研究的目的:
- 研究let-60ras基因在阴道发育中的功能.
- 为了确定let-60ras蛋白的区域,该蛋白对其激活和功能至关重要.
主要方法:
- 在let-60基因中对衰退性和主导性突变的分析.
- 无阴道和多阴道表型的特征.
- 检查let-60基因在其遗传途径中的作用.
主要成果:
- 递归突变会损害let-60在响应诱导信号时的功能,突出区域进行激活和作用.
- 主导突变改变子13激活let-60独立于诱导信号.
- let-60在一个涉及调节分子和点分子的遗传途径中运作.
结论:
- 莱特-60ras基因是C. elegans*阴道形成的关键调节者.
- 莱特-60ras蛋白的特定区域对于信号响应和激活至关重要.
- 让-60路径涉及复杂的监管和下游目标,对发展至关重要.
相关概念视频
The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
Small GTPases - Ras and Rho
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...


