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在先天性心脏病患者心脏前体细胞的特征和功能

Rachana Mishra¹, Kalpana Vijayan, Evan J Colletti

¹Children's Memorial Hospital, Division of Cardiovascular-Thoracic Surgery, 2300 Children's Plaza, Chicago, IL 60614, USA.

|January 19, 2011

概括

人类心脏原生细胞 (hCPC) 在新生儿中最为丰富,并且随着年龄的增长而减少. 这些细胞被分离为心脏球衍生细胞 (hCDCs),显示出对先天性心脏缺陷修复的潜力.

科学领域:

心血管研究研究心血管研究
再生医学是一种再生医学.
发育生物学发展生物学

背景情况:

人类心脏原生细胞 (hCPC) 显示出心肌再生的前景.
在儿科先天性心脏缺陷修复中hCPCs的潜力需要研究.

研究的目的:

在患有先天性心脏缺陷的年轻患者中探索hCPCs的再生能力.
为了研究hCPC丰度和功能的年龄相关变化.

主要方法:

人的右心房样本从新生儿,婴儿和接受心脏手术的儿童中收集.
使用免疫光学 (Ki67,NKX2-5,NOTCH1,NUMB) 量化了C-kit(+) 的hCPC.
使用心脏圈方法分离和扩展右心室衍生的CPCs,形成人类心脏圈衍生的细胞 (hCDCs).

主要成果:

新生儿的hCPCs显著高于老年儿童,新生儿的hCPCs显著高于老年儿童,新生儿期的峰值增殖和分化.
从所有儿科年龄组中成功分离和扩展hCDC.
hCDCs证明了多种心血管系的分化,并在体内促进心肌再生.

结论:

居民hCPC在新生儿中最为丰富,并且随着年龄的增长而下降.
hCDC是用于儿科先天性心脏修复的可行细胞来源,无论年龄或诊断如何.
hCPC 具有用于心脏修复应用的功能再生能力.

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