GAL11P:一种酵母突变,增强弱GAL4衍生激活剂的作用
H J Himmelfarb1, J Pearlberg, D H Last
1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Cell
|December 21, 1990
概括
在酵母中,一种特定的GAL11 (SPT13) 突变增强了GAL4激活剂的活性. 这表明,GAL4-GAL11复合体形成了一个强大的激活剂,可能在DNA上起作用.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 转录条例 转录条例 转录条例
背景情况:
- 在酵母中,GAL4是关键的转录激活剂.
- 已知GAL11 (SPT13) 能调节GAL4的活动.
- 了解激活剂-协同激活剂相互作用对于基因调节至关重要.
研究的目的:
- 研究GAL11在调节GAL4转录活性中的作用.
- 为了表征一种特定的GAL11突变,增强GAL4功能.
- 为了阐明GAL4-GAL11相互作用的机制.
主要方法:
- 酵母突变菌的遗传分析.
- 构建 GAL4 和 GAL11 融合蛋白.
- 对转录激活的测试.
主要成果:
- 在GAL11中,单个误解突变将GAL4衍生的弱活化剂转化为强活化剂.
- 化到酸性激活序列的GAL4的DNA结合域对GAL11和突变的GAL11都作出反应.
- 当它与DNA结合时,GAL11本身就起到强大的激活作用,其被删除会降低GAL4的活性.
结论:
- 一个GAL4和GAL11复合体形成了一个特别强大的转录激活剂.
- 有证据表明,GAL4-GAL11复合体主要在DNA上形成,为GAL11的功能提供了生物学理由.
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