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相关概念视频

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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相关实验视频

Updated: Jun 5, 2026

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

一个 aldehyde微阵列用于高通量剖析细胞事件的.

Hao Wu1, Jingyan Ge, Peng-Yu Yang

  • 1Department of Chemistry, 3 Science Drive 3, National University of Singapore, Singapore 117543.

Journal of the American Chemical Society
|January 21, 2011
PubMed
概括

我们开发了一种小分子微阵列 (SMM),用于分析复杂生物样本中的酶活性. 这种有针对性的方法成功地在各种细胞和感染样本中识别了囊蛋白酶及其活性.

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物化学 生物化学
  • 酶学 是一种酶学.

背景情况:

  • 微阵列为大规模的生物分析提供了强大的工具.
  • 了解本地样本中的酶活性对于生物研究至关重要.

研究的目的:

  • 开发一种小分子微阵列 (SMM) 用于分析酶活性.
  • 为了能够在复杂样本中对囊蛋白酶进行大规模的功能评估.

主要方法:

  • 合成了270种酶抑制剂的聚焦位置扫描库.
  • 基于化的抑制剂被固定在阿维丁片上,以创建SMM.
  • 该SMM用于分析纯蛋白质,细胞溶解物和受感染的样本.

主要成果:

  • 该SMM专门针对囊蛋白酶,显示与模型蛋白质一致的结合指纹.
  • 清晰的蛋白酶活性特征从亡的Hela细胞和感染Plasmodium falciparum的红细胞中获得.
  • 该SMM在红细胞溶解体内区分了寄生虫感染阶段.
  • 假定蛋白质标记物被识别并使用从微阵列中获得的探针进行验证.

结论:

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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

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Published on: January 7, 2019

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

  • 向的SMM在复杂的蛋白质样本中剖析细胞生物学方面是有效的.
  • 这种方法促进了大规模的功能评估和酶活性的发现.
  • SMM技术对目标验证和生物标志物发现有潜力.