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相关概念视频

Transcription01:10

Transcription

155.9K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
155.9K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.6K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.6K
Transcription Elongation Factors02:35

Transcription Elongation Factors

13.6K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.6K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.2K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.2K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

10.9K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
10.9K
Master Transcription Regulators02:23

Master Transcription Regulators

7.7K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K

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相关实验视频

Updated: Jan 27, 2026

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
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Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

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新生转录序列化可视化了核酸分辨率上的转录.

L Stirling Churchman1, Jonathan S Weissman

  • 1Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, USA.

Nature
|January 21, 2011
PubMed
概括
此摘要是机器生成的。

原生延长转录序列 (NET-seq) 监测核酸分辨率的RNA聚合酶活性. 这种方法揭示了Rpd3S复合物如何控制转录方向性,以及核细胞如何通过导致聚合酶暂停来阻止延长.

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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
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Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
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Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants

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Transcriptional Analysis by Nascent RNA FISH of In Vivo Trophoblast Giant Cells or In Vitro Short-term Cultures of Ectoplacental Cone Explants
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物物理学的生物物理.

背景情况:

  • 转录调节涉及复杂的启动后控制和快速的转录退化.
  • 了解转录生成需要追踪新生RNA分子的方法.
  • 之前的方法缺乏核酸分辨率跟踪活跃转录.

研究的目的:

  • 开发和应用一种用于监测核酸分辨率的新生转录的新方法.
  • 调查Rpd3S复合体在控制主办方位方面的作用.
  • 在转录延长过程中识别和描述聚合酶暂停和逆行.

主要方法:

  • 开发了原生延长转录序列 (NET-seq),利用与RNA聚合酶相关的新生转录的3'端的深度序列.
  • 用NET-seq对Saccharomyces cerevisiae进行了应用,以分析转录动态.
  • 计算分析被用来识别促销者方向性,暂停和回溯站点.

主要成果:

  • NET-seq提供了转录的核酸分辨率监测.
  • 通过抑制反意义转录,Rpd3S脱乙烯化复合体通过抑制反意义转录来强制促进子方向性.
  • 无处不在的聚合酶暂停和回溯在整个转录中发生,在核细胞上达到峰值.
  • 核子体诱导的暂停在体内作为转录延长的重要障碍.

结论:

  • NET-seq是研究转录动态的一个强大的工具.
  • 在建立单向转录方面,Rpd3S复合体起着至关重要的作用.
  • 核细胞显著阻碍转录延长通过暂停,突出一个关键的调节机制.