人类ES和iPS细胞变异的参考地图使多能细胞系的高通量表征成为可能
Christoph Bock1, Evangelos Kiskinis, Griet Verstappen
1Broad Institute, Cambridge, MA 02142, USA.
Cell
|February 8, 2011
概括
人类多能干细胞 (ES和iPS细胞) 显示出显著的变异,影响研究效用. 这项研究绘制了它们的表观遗传学和基因表达的地图,以预测分化效率,创建一个表征得分卡.
科学领域:
- 干细胞生物学 干细胞生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 人类多能干细胞,包括胚胎干细胞 (ES) 和诱导多能干细胞 (iPS),有望产生与疾病相关的细胞类型.
- 不同的多能细胞系之间存在显著的变异性,这可能会影响它们的研究应用和临床安全.
- 对细胞系特异性的更深入的理解对于可靠的翻译研究至关重要.
研究的目的:
- 建立人类ES和iPS细胞系的DNA甲基化和基因表达的综合性全基因组参考地图.
- 评估ES和iPS细胞之间的表观遗传和转录相似性和差异.
- 开发一种方法来预测多能细胞系的体外分化倾向.
主要方法:
- 为20个人类ES和12个人类iPS细胞系生成全基因组DNA甲基化和基因表达参考图.
- 在体外分化试验测量这些细胞系的分化潜力.
- 生物信息分析用于比较表观遗传和转录特征,并将它们与差异化效率相关联.
主要成果:
- 为大量人类多能干细胞队列建立了参考表观基因组和转录基因组图.
- 证明了这些地图对评估ES和iPS细胞之间的相似性的有用性.
- 基于单个细胞系的分子形状,开发了单个细胞系的差异化效率的预测模型.
结论:
- 开发的资源为多能细胞系的全面表征提供了一个得分表.
- 了解细胞系特定的表观遗传和转录景观是预测和优化它们的分化潜力的关键.
- 这项工作有助于在生物医学研究和翻译应用中可靠地使用人类ES和iPS细胞.
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