RIP3调解了caspase-8缺乏的小鼠胚胎死亡率
William J Kaiser1, Jason W Upton, Alyssa B Long
1Department of Microbiology and Immunology, Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA. wkaiser@emory.edu
Nature
|March 4, 2011
概括
卡斯帕-8 (Casp8) 通常可以抑制亡. 缺少Casp8和RIP3的双突变小鼠是可活的,这表明这些蛋白质对于发育是不可或缺的,但对于免疫平衡至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
- 发育生物学是发展生物学.
背景情况:
- 卡斯帕-8 (Casp8) 对亡至关重要,并调节亡.
- Casp8还影响天生的免疫力和细胞分化.
- 卡斯普8缺乏导致胚胎死亡,这表明它在发育中起作用.
研究的目的:
- 研究Casp-8 (Casp8) 和RIP3在哺乳动物发育和免疫功能中的作用.
- 为了确定RIP3是否在Casp8缺乏的小鼠中调解胚胎死亡率.
- 探索Casp8和RIP3在成人免疫恒温的联合功能.
主要方法:
- 产生和分析Casp8(-/-)Rip3(-/-) 双淘汰赛小鼠.
- 双重突变小鼠的表型特征,包括生存能力,生育能力和免疫细胞种群.
- 评估免疫系统功能和识别与年龄相关的疾病,如淋巴腺病变.
主要成果:
- 在Casp8缺乏的小鼠中,RIP3缺乏挽救了胚胎死亡率,显示RIP3调解了这种死亡率.
- Casp8(-/-) Rip3(-/-) 双重突变小鼠是可活的,肥沃的,并且具有正常的髓状和淋巴状细胞补充.
- 尽管看似具有免疫能力,但由于异常的T细胞积累,双重突变小鼠会患上淋巴腺病,这表明它们在免疫平衡中发挥了作用.
结论:
- 当它们一起作用时,Casp-8 (Casp8) 和RIP3对于哺乳动物的发育是不可或缺的.
- Casp8和RIP3在维持成人免疫系统平衡中发挥着关键的,尽管是不同的作用.
- Casp8和RIP3之间的相互作用对于防止异常T细胞积累和淋巴腺病变至关重要.
相关概念视频
01:24Caspases
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.
01:17The Extrinsic Apoptotic Pathway
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...