磁纳米粒子 - - 蛋白质子单元的模板组装:基于碳水化合物的MRI纳米探针的新平台
Elsa Valero1, Stefano Tambalo, Pasquina Marzola
1Departamento de Química Inorgánica, Universidad de Granada, 18071 Granada, Spain.
Journal of the American Chemical Society
|March 10, 2011
概括
研究人员开发了碳水化合物涂层的磁性纳米粒子,使用阿波费里丁用于增强磁共振成像 (MRI) 对比剂. 这些新的Apomaghemite纳米颗粒显示出医学成像应用的前景.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 生物医学成像技术 生物医学成像技术
背景情况:
- 阿波费里丁蛋白的pH驱动的组装-拆卸可以封装磁性纳米粒子.
- 阿波费里丁上的功能性氨基团使碳水化合物结合成为可能.
研究的目的:
- 为MRI应用准备碳水化合物涂层的磁性纳米粒子 (Apomaghemites).
- 描述这些纳米粒子并评估它们作为MRI对比剂的性能.
- 为了评估特定的碳水化合物-乳素结合亲和力的保留.
主要方法:
- 通过pH驱动的组装将maghemite纳米颗粒封装在apoferritin中.
- 阿波费里丁与N-乙-D-葡萄糖胺和d-曼诺糖的功能化.
- 使用高分辨率电子显微镜 (HREM),HAADF-STEM,EELS,XRD和SQUID进行了表征.
- 在体内评估MRI对比度增强和放松性 (r2).
- 评估碳水化合物-乳素结合亲和力.
主要成果:
- 成功合成了碳水化合物功能化的磁性纳米粒子 (Apomaghemites).
- 阿波马赫米特显示MRI对比度增强与商业药物 (Endorem) 相比,具有显著的r2放松性.
- 碳水化合物功能化保留了特定的莱克结合能力.
结论:
- 阿波马盖米特是有效的MRI对比剂,具有临床使用的潜力.
- 碳水化合物涂层保持了特定的生物识别特性.
- 这种方法为开发有针对性的诊断工具提供了一种新方法.
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