在金属单壁碳纳米管网络上支持酶的高性能生产和氧化电极
Draženka Svedružić1, Jeffrey L Blackburn, Robert C Tenent
1Biosciences Center, National Renewable Energy Laboratory, 1617 Cole Boulevard, Golden, Colorado 80401, USA.
Journal of the American Chemical Society
|March 10, 2011
概括
我们通过将其固定在金属单壁碳纳米管 (m-SWNT) 网络上来增强[FeFe]-酶 (CaH2ase) 的电催化活性. 这改善了质子还原和氧化,显示了生物电触媒的潜力.
科学领域:
- 生物电催化剂生物电催化剂
- 纳米材料科学 科学 纳米材料科学
- 酶固定化 酶固定化
背景情况:
- [FeFe] - 基酶是代谢的关键酶.
- 有效的固定是增强酶电催化活性的关键.
- 碳纳米材料为酶支持提供独特的特性.
研究的目的:
- 为了研究Clostridium acetobutylicum [FeFe]-酶 (CaH2ase) 的电催化性能.
- 评估单壁碳纳米管 (SWNT) 网络组成对CaH2酶活性的影响.
- 了解金属SWNT (m-SWNT) 在提高生物电催化效率中的作用.
主要方法:
- 在碳布上将CaH2酶固定在SWNT网络上,具有不同的金属和半导体比率.
- 使用超声波喷雾来准备SWNT网络.
- 质子还原和氧化活动的电化学表征.
主要成果:
- 在富含m-SWNT的网络上固定的CaH2酶显示电流密度至少增加了10倍.
- 对于质子减少和氧化都观察到增强的活性.
- 在低m-SWNT含量或缺少SWNT的情况下没有显著的活性增强.
结论:
- 金属SWNT显著提高了CaH2酶的电催化活性.
- 增加活性电极表面积和改进的电子合是关键因素.
- m-SWNT网络是高效生物电催化应用的有希望的支持.
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