Pseudomonas sax 基因克服了 Arabidopsis 中的非宿主异酸盐介导的异形性耐药性
Jun Fan1, Casey Crooks, Gary Creissen
1John Innes Centre, Norwich NR4 7UH, UK. jun.fan@bbsrc.ac.uk
Science (New York, N.Y.)
|March 10, 2011
概括
天然植物化合物如硫福拉 (4-甲基硫inylbutyl isothiocyanate) 是对抗细菌病原体的关键防御. 特定的细菌基因 (sax基因) 帮助病原体克服这些植物防御,导致疾病.
科学领域:
- 植物病理学 植物病理学
- 自然产品化学 自然产品化学
- 微生物学 微生物学
背景情况:
- 大多数植物微生物相互作用都是非致病性的.
- 自然产品在植物对病原体的防御中起着重要作用.
研究的目的:
- 研究天然产品硫福拉在植物对非宿主细菌的防御中的作用.
- 为了确定参与克服植物防御的细菌因素.
主要方法:
- 在植物生长抑制测定Pseudomonas细菌在Arabidopsis.
- 鉴定和描述Pseudomonas sax基因.
- 对 Pseudomonas 菌株进行基因操纵,以引入或删除 sax 基因.
主要成果:
- 硫福拉 (4-甲基硫inylbutyl isothiocyanate) 抑制了Arabidopsis中非宿主Pseudomonas细菌的生长.
- Pseudomonas sax 基因 (saxCAB/F/D/G) 对于毒性菌株来说是必要的,以克服异酸盐防御.
- 将saxCAB基因引入非宿主菌株,赋予了克服植物防御的能力.
结论:
- 亚利法性异硫酸酸盐,包括硫福拉,是植物对病原体的防御中至关重要的.
- 细菌的萨克斯基因对于毒性至关重要,因为它们使病原体能够逃避基于异硫酸的抗性.
- 这些发现强调了天然产品在非宿主耐药性和病原体毒性的遗传基础中的重要性.
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