罗多普辛在Drosophila的温度歧视中的作用
Wei L Shen1, Young Kwon, Abidemi A Adegbola
1Department of Biological Chemistry, Center for Sensory Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Science (New York, N.Y.)
|March 12, 2011
概括
果幼虫使用一种特定的温度选择途径,涉及G蛋白,脂酶C和TRPA1通道. 罗多普辛是一种与G蛋白结合的受体,对于这种光独立的热电作用至关重要.
科学领域:
- 动物行为 动物行为
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 动物表现出热敏度,果 (Drosophila) 显示出精确的温度选择.
- 草幼虫的热毒作用依赖于涉及G蛋白,脂酶C和TRPA1通道的途径.
- 在此前,G蛋白合受体 (GPCRs) 在这种热传感途径中的特定作用尚不清楚.
研究的目的:
- 为了研究多索菲拉罗多普辛 (被ninaE编码) 在热触性行为中的作用.
- 为了确定罗多普辛在热阻力中的功能是否依赖光.
- 探索热传感中保存GPCR功能的潜力.
主要方法:
- 在Drosophila幼虫中 ninaE基因的基因突变.
- 在舒适的温度下进行行为测试,以评估热触差别.
- 在突变幼虫中使用老鼠黑色素的功能性救援实验.
主要成果:
- 编码罗多素的 ninaE 基因发生突变,消除了 Drosophila 幼虫的热触性歧视.
- 这种热阻力缺陷发生在不受光照影响的情况下.
- 引入小鼠黑色素恢复了 ninaE 突变幼虫的正常热触行为.
结论:
- 草 rhodopsin 在启动热感应信号的热毒性方面发挥着关键的,独立于光的作用.
- 罗多平素代表了一类保存的GPCRs,它们对热传感途径至关重要.
- 这一发现将GPCRs已知的功能扩展到光传导之外.
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