MPF的环林B2成分是c-mos(xe) 原型瘤基因产品的基质
1Department of Pharmacology, University of Colorado School of Medicine, Denver 80262.
Cell
|June 1, 1990
概括
莫斯原型瘤基因直接酸化环素B2,这是成熟促进因子 (MPF) 的关键成分. 这一发现揭示了在卵细胞成熟期间MPF激活的新型机制,涉及mos介导的环林B2酸化.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 发展生物学 发展生物学
- 瘤基因是一种瘤基因.
背景情况:
- 成熟促进因子 (MPF) 对于卵细胞的成熟至关重要.
- MPF包括与cdc2激酶复合的环林B2.
- 激活MPF需要c-mos原型瘤基因.
研究的目的:
- 调查c-mos原瘤基因在MPF激活中的作用.
- 为了确定莫斯是否直接酸化环素B2.
- 为了阐明肌肉中介MPF激活的机制.
主要方法:
- 在体外酸化试验中,使用免疫沉的v-mos和c-mos.
- 聚分析以比较酸化模式.
- 在卵细胞中注射反感性寡核酸来消去c-mos (x) 细胞.
主要成果:
- 病毒性mos (v-mos) 和Xenopus c-mos (c-mos(xe)) 在体外直接化环素B2.
- 莫斯的酸化模式与cdc2激酶的酸化模式相似.
- 切除c-mos (xe) 降低了卵细胞提取物中环林B2酸化的40%.
结论:
- 这种c-mos原型瘤基因直接酸化环素B2.
- 摩斯介导的环林B2酸化是卵细胞成熟期间MPF激活的一个关键步骤.
- 这项研究揭示了MPF激活的新型机制,涉及菌对环林成分的直接酸化.
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